Biochemical methods / S. Sadasivam, A. Manickam.

Biochemical methods are used in all branches of biological sciences and biotechnology. Biochemical experiments are conducted in the laboratory as practicals as well as for pursuing research. A researcher has to refer to many journals and books before he/she could get to the working protocol for his/her experiment. This book attempts to give often-used methods in a single volume. This edition of ‘Biochemical Methods’ is divided into 13 chapters. Each experiment includes introduction, principle, materials, procedure and calculation. At the end of each procedure, references for additional reading are provided. Important precautions, warnings and tips are given under the Notes section. In addition, there are seven Appendices, which give minute details of basic chemistry, buffer preparations, Reverse Transcription Polymerase Chain Reaction (RT/PCR) and other aspects required for the conduction of the experiments. The methods given in the book will be useful for conducting practical classes at the undergraduate and postgraduate levels in biochemistry, biotechnology, microbiology, agricultural science, environmental science, botany, zoology, nutrition, pharmaceutical science and other biology-related subjects. This book will be a bonanza for the research workers since it covers procedures from the classical Micro Kjeldahl Nitrogen estimation to the modern PCR technique.